Plasmid

Part:BBa_J100268:Design

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)


pJC184d5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal XbaI site found at 7731
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal BglII site found at 3415
    Illegal BglII site found at 6176
    Illegal BamHI site found at 2024
    Illegal BamHI site found at 2293
    Illegal BamHI site found at 4434
    Illegal BamHI site found at 6963
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal XbaI site found at 7731
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal XbaI site found at 7731
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
    Illegal AgeI site found at 1859
    Illegal AgeI site found at 2165
    Illegal AgeI site found at 2654
    Illegal AgeI site found at 2852
    Illegal AgeI site found at 3313
    Illegal AgeI site found at 3536
    Illegal AgeI site found at 4709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5828
    Illegal SapI site found at 1841
    Illegal SapI.rc site found at 3503


Design Notes

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014


Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

References